Biochemistry, Clinical & Medical Chemistry Calculators
All biochemistry, clinical & medical chemistry calculators in one place.
Blood Alcohol Concentration (BAC) Calculator
Estimate your Blood Alcohol Concentration from your drinks, weight, and time using the Widmark formula, with impairment stage and sober-up time.
Anion Gap & Serum Osmolar Gap Solver
Calculate the anion gap (with albumin correction) and serum osmolar gap from your basic metabolic panel, with instant interpretation.
Protein Mass & Peptide Molecular Weight
Calculate the average and monoisotopic molecular weight of any peptide or protein from its amino acid sequence, estimate its UV extinction coefficient and back out concentration from an A280 reading, and convert freely between mass, volume and molar concentration for dosing, dilution and reconstitution, with an amino acid composition chart and complete step-by-step working.
DNA / RNA Concentration & Yield Solver
Calculate DNA or RNA concentration from an A260 reading with automatic dsDNA/ssDNA/RNA conversion factors, check A260/A280 and A260/A230 purity ratios, work out total yield and molar concentration, find copy number for qPCR, and build a full dilution series for a qPCR standard curve, with complete step-by-step working.
Oligonucleotide Melting Temperature
Estimate primer/probe melting temperature (Tm) from a DNA or RNA sequence using the Wallace rule, the %GC method, and full nearest-neighbor thermodynamics (SantaLucia 1998), with a salt-corrected result and complete step-by-step working.
Resuscitation Fluid / Electrolyte Deficit
Calculate maintenance IV fluid rate (Holliday-Segar), fluid deficit from % dehydration, sodium and potassium deficits, an emergency resuscitation bolus, and a full 24-hour replacement schedule, with complete step-by-step working.
Blood pH & Henderson-Hasselbalch Blood Gas
Calculate blood pH from PaCO2 and HCO3 using the Henderson-Hasselbalch equation, identify respiratory or metabolic acidosis/alkalosis, and check compensation with Winter's formula and standard ABG rules.
Ligand Binding Kinetics & Dissociation (Kd)
Find the dissociation constant (Kd) from kon and koff or enter it directly, calculate equilibrium occupancy and bound fraction for any free ligand concentration, model association kinetics (kobs, half-time to equilibrium) with a binding curve, and work out dissociation half-life and residence time after washout, with full step-by-step working.
Isothermal Titration Calorimetry (ITC) Solver
Find Ka, Kd, ΔG and ΔS from a measured ΔH and temperature, check the Wiseman c-value to see whether a planned ITC experiment will give a well-shaped binding curve (with a reverse lookup for the ideal cell concentration), and simulate a single-site Wiseman binding isotherm with cumulative heat per injection, with full step-by-step working and a binding curve chart.
Enzyme Inhibition (Ki) Solver
Convert IC50 to the true inhibition constant Ki with the Cheng-Prusoff equation (and back again), solve for Ki from a single inhibited velocity reading with apparent Km'/Vmax', and calculate percent inhibition or a full IC50 dose-response curve — for competitive, noncompetitive, and uncompetitive inhibitors, with full step-by-step working.
SDS-PAGE Protein Migration Distance
Calculate relative mobility (Rf) from migration distance, build a log(MW) vs Rf calibration curve from your marker standards to estimate an unknown protein's molecular weight (or predict migration distance from a known MW), and get a resolving-gel percentage recommendation, with full step-by-step working and a calibration curve chart.